Persönlicher Status und Werkzeuge

Sprachwahl

Equipment

  • Plasmid cloning

    • PCR
    • library construction
    • site-directed mutagenesis
    • automatic sequencing  

  • Bacterial protein expression systems

  • Protein purification and detection

    • electrophoresis, Western blotting, densitometry
    • UV/VIS- spectroscopy (cuvette and 96-well format)
    • HPLC, FPLC

  • Protein/Protein interaction

    • ToxR/POSSYCCAT system
    • Enzyme assays
    • ultracentrifugation
    • Liposome-Liposome fusion
    • fluorescence spectroscopy (cuvette and 96-well format)

  • Protein and peptide structure analysis

    • Circular dichroism spectroscopy
    • Hydrogen/Deuterium exchange
    • ESI-IMS-TOF tandem mass spectrometry (ESI-Q-TOF, MS or MS/MS with ETD and ion mobility mass spectrometry function (Synapt G2, Waters)
    • LC/MS ESI-TOF mass spectrometry (Agilent ToF)